解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Prediction of tissue damage under thermal loads plays important role for thermal ablation planning. A new methodology is presented in this paper by combing non-Fourier bio-heat transfer, constitutive elastic mechanics as well as non-rigid motion of dynamics to predict and analyze thermal distribution, thermal-induced ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1227609
更新日期:2017-01-02 00:00:00
abstract::Touch is produced by sensations that include approaching, sliding, pressing, and temperature. This concept has become a target of research in biotechnology, especially in the field of bionic biology. This study measured sliding and pressing with traditional tactile sensors in order to improve a machine operator's judg...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1227612
更新日期:2017-01-02 00:00:00
abstract::The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone gro...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1227141
更新日期:2017-01-02 00:00:00
abstract::Botulinum toxin type A can temporarily inhibit muscle contraction. Currently, physicians administer this toxin as a bio-drug in treatment of some muscle contraction disorders. TAT-BoNT/A(1-448) is a functional recombinant protein derived from botulinum toxin light chain. Unlike the full length botulinum toxin, TAT-BoN...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1201252
更新日期:2016-11-01 00:00:00
abstract::A genetic recombinant Saccharomyces cerevisiae starter with high ethanol tolerance capacities was constructed. In this study, the gene of trehalose-6-phosphate synthase (encoded by tps1), which catalyzes the first step in trehalose synthesis, was cloned and overexpressed in S. cerevisiae. Moreover, the gene of neutral...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1207019
更新日期:2016-11-01 00:00:00
abstract::For somatic gene therapy of hemophilia B, hepatocytes as the main cellular host for expression of hFIX are attractive targets. In gene therapy protocols, an efficient expression vector equipped with cis-regulatory elements such as introns is required. With this in mind, hFIX-expressing plasmids equipped with different...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1207018
更新日期:2016-11-01 00:00:00
abstract::The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in nor...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2016.1227143
更新日期:2016-09-02 00:00:00
abstract::We propose a mechanism of action for the betL* mutation which is based on DNA topology. Removing a single thymine residue from the betL σ(A) promoter's -10 and -35 spacer results in a 'twist'-mediated activation of transcription which accounts for the osmotolerance phenotype observed for strains expressing betL*. ...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.1080/21655979.2016.1171438
更新日期:2016-04-02 00:00:00
abstract::CRISPR (Clustered Regularly-Interspaced Short Palindromic Repeats)-Cas9 (CRISPR associated protein 9) has rapidly become the most promising genome editing tool with great potential to revolutionize medicine. Through guidance of a 20 nucleotide RNA (gRNA), CRISPR-Cas9 finds and cuts target protospacer DNA precisely 3 b...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2016.1189039
更新日期:2016-04-01 00:00:00
abstract::Quorum sensing (QS) is a prevalently found intercellular signaling system in bacteria. QS system bestows behavioral coordination ability in bacteria at high population density. QS via acylated homoserine lactone (AHL) is extensively conserved in Gram-negative bacteria and plays crucial role in regulating many biologic...
journal_title:Bioengineered
pub_type: 评论,杂志文章
doi:10.1080/21655979.2016.1174797
更新日期:2016-04-01 00:00:00
abstract::Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased towa...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1128589
更新日期:2016-01-01 00:00:00
abstract::The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice. We showed evidences of the biotechnological potential of the ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1131368
更新日期:2016-01-01 00:00:00
abstract::Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-added products through microbial fermentation. Both anaerobic fungi and anaerobic bacteria have evolved to produce large multi-cellulase complexes referred to as cellulosomes, which are powerful machines for biomass deconstruct...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2015.1060379
更新日期:2015-01-01 00:00:00
abstract::We report the molecular cloning, expression, and single-step homogeneous purification of RNA polymerase II subunit RPB5 from Saccharomyces cerevisiae. RPB5 is a 210 amino acid nuclear protein that functions as the fifth largest subunit of polymerase II and plays a central role in transcription. The gene that codes for...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2014.1002301
更新日期:2015-01-01 00:00:00
abstract::In this paper, Penaeus orientolis prawn freshness rapid determination method using electronic nose (e-nose) and non-linear data processing technique is studied. E-nose responses to prawns stored at 4 °C are measured. Meanwhile, physical/chemical indexes (firmness, pH, total volatile basic nitrogen (TVB-N), total viabl...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/21655979.2014.993294
更新日期:2015-01-01 00:00:00
abstract::In this paper, a miniaturized cell suspension concentration monitoring method was investigated. The sensing unit was a carbon screen-printed electrode (CSPE) in serial with a 433MHz vacuum-packaged surface acoustic wave resonator (SAWR). SAWR provided a stable and high operating frequency, which helps to keep the stab...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1119342
更新日期:2015-01-01 00:00:00
abstract::Organ transplantation can offer a curative option for patients with end stage organ failure. Unfortunately the treatment is severely limited by the availability of donor organs. Organ bioengineering could provide a solution to the worldwide critical organ shortage. The majority of protocols to date have employed the u...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.1080/21655979.2015.1081320
更新日期:2015-01-01 00:00:00
abstract::We previously presented the cloning, heterologous expression, and characterization of a novel multidomain endoxylanase from Arthrobacter sp. GN16 isolated from the feces of Grus nigricollis. Molecular and biochemical characterization studies indicate that the glycoside hydrolase (GH) family 10 domain at the N-terminus...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2014.1004021
更新日期:2015-01-01 00:00:00
abstract::The detecting mechanism of carbon nanotubes gas sensor based on multi-stable stochastic resonance (MSR) model was studied in this paper. A numerically stimulating model based on MSR was established. And gas-ionizing experiment by adding electronic white noise to induce 1.65 MHz periodic component in the carbon nanotub...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1058453
更新日期:2015-01-01 00:00:00
abstract::D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals. However, the biochemical properties of YjhG still remain unknown. In this study, the activity assay method for YjhG was estab...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.1080/21655979.2015.1040208
更新日期:2015-01-01 00:00:00
abstract::During the last few decades, Streptomycetes have shown to be a very important and adaptable group of bacteria for the production of various beneficial secondary metabolites. These secondary metabolites have been of great interest in academia and the pharmaceutical industries. To date, a vast variety of techniques and ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.29935
更新日期:2014-09-01 00:00:00
abstract::Lon protease is conserved from bacteria to humans and regulates cellular processes by degrading different classes of proteins including antitoxins, transcriptional activators, unfolded proteins, and free ribosomal proteins. Since we found that Lon has several putative cyclic diguanylate (c-di-GMP) binding sites and si...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.29261
更新日期:2014-07-01 00:00:00
abstract::Mammalian collagen has been widely used as a biomedical material. Nevertheless, there are still concerns about the variability between preparations, particularly with the possibility that the products may transmit animal-based diseases. Many groups have examined the possible application of bioengineered mammalian coll...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.28791
更新日期:2014-07-01 00:00:00
abstract::We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also r...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.27607
更新日期:2014-05-01 00:00:00
abstract::Due to the limited self-renewal capacity of cardiomyocytes, the mammalian heart exhibits impaired regeneration and insufficient ability to restore heart function after injury. Cardiovascular tissue engineering is currently considered as a promising alternative therapy to restore the structure and function of the faili...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.27751
更新日期:2014-05-01 00:00:00
abstract::Herein, I track the evolution of synthetic biology from its earliest incarnations more than 50 years ago, through the DIYbio revolution, to the next 50 years. ...
journal_title:Bioengineered
pub_type: 社论
doi:10.4161/bioe.28317
更新日期:2014-03-01 00:00:00
abstract::The influenza A virus is a critical public health problem that causes epidemics and pandemics, and occurs widely all over the world. Various vaccines against the virus have not provided a solution to the problem. Different approaches, particularly M2e peptide-based vaccines, are available for developing universal vacc...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/21655979.2014.969131
更新日期:2014-01-01 00:00:00
abstract::Bovine chymosin is an important milk-clotting agent used in the manufacturing of cheeses. Currently, the production of recombinant proteins by genetically modified organisms is widespread, leading to greatly reduced costs. Lactococcus (L.) lactis, the model lactic acid bacterium, was considered a good candidate for he...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.36327
更新日期:2014-01-01 00:00:00
abstract::This paper investigates the application of anodic porous alumina as an advancement on chip laboratory for gene expressions. The surface was prepared by a suitable electrolytic process to obtain a regular distribution of deep micrometric holes and printed bypen robot tips under standard conditions. The gene expression ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.25278
更新日期:2013-09-01 00:00:00
abstract::Biodiesel, as one type of renewable energy, is an ideal substitute for petroleum-based diesel fuel and is usually made from triacylglycerides by transesterification with alcohols. Biodiesel production based on microbial fermentation aiming to establish more efficient, less-cost and sustainable biodiesel production str...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.23114
更新日期:2013-09-01 00:00:00
abstract::This invited commentary covers the period 1997-2012 and has seen changes in terminology that progressed from "basic" and "applied" to "translational" research. In the context of Bioengineered, these changes map readily onto the processes of identifying microbial characteristics appropriate for specific applications, i...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.23251
更新日期:2013-07-01 00:00:00
abstract::Illicium verum (badiane or star anise), Crataegus oxyacantha ssp monogyna (hawthorn) and Allium cepa (onion), have traditionnally been used as medicinal plants in Algeria. This study showed that the outer layer of onion is rich in flavonols with contents of 103 ± 7.90 µg/g DW (red variety) and 17.3 ± 0.69 µg/gDW (whit...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.24435
更新日期:2013-07-01 00:00:00
abstract::Microbial enzymes have been used in a large number of fields, such as chemical, agricultural and biopharmaceutical industries. The enzyme production rate and yield are the main factors to consider when choosing the appropriate expression system for the production of recombinant proteins. Recombinant enzymes have been ...
journal_title:Bioengineered
pub_type: 杂志文章,评审
doi:10.4161/bioe.24761
更新日期:2013-07-01 00:00:00
abstract::The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22818
更新日期:2013-07-01 00:00:00
abstract::The anti-inflammatory cytokine interleukin-10 and its viral homologs were chosen as model proteins for the development of drug delivery systems based on probiotic carriers like E. coli Nissle 1917, E. coli G3/10, and Saccharomyces boulardii. Exterior cytokine secretion was achieved by a modified E. coli hemolysin tran...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22646
更新日期:2013-05-01 00:00:00
abstract::Engineered zinc-finger nucleases (ZFNs) are powerful tools for creating double-stranded-breaks (DSBs) in genomic DNA in a site-specific manner. These DSBs generated by ZFNs can be repaired by homology-directed repair or nonhomologous end joining, in which the latter can be exploited to generate insertion or deletion m...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22262
更新日期:2013-03-01 00:00:00
abstract::A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.22350
更新日期:2013-03-01 00:00:00
abstract::Bacterial L-asparaginase has been a universal component of therapies for childhood acute lymphoblastic leukemia since the 1970s. Two principal enzymes derived from Escherichia coli and Erwinia chrysanthemi are the only options clinically approved to date. We recently reported a study of recombinant L-asparaginase (Ans...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.21710
更新日期:2013-01-01 00:00:00
abstract::Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker's yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to th...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.21444
更新日期:2012-11-01 00:00:00
abstract::Protein production through dedicated secretion systems might offer an potential alternative to the conventional cytoplasmical expression. The application of Type 1 secretion systems of Gram-negative bacteria, however, where often not successful in the past for a wide range of proteins. Recently, two studies using the ...
journal_title:Bioengineered
pub_type: 杂志文章
doi:10.4161/bioe.20712
更新日期:2012-09-01 00:00:00